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maxpar cell staining buffer  (fluidigm)


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    Structured Review

    fluidigm maxpar cell staining buffer
    Maxpar Cell Staining Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 713 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+staining+buffer/Maxpar+Cell+Staining+Buffer/pmc13043783-80-10-16
    Average 96 stars, based on 713 article reviews
    maxpar cell staining buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: An integrated multi-omic natural history study of human development, sexual dimorphism, and the effects of trisomy 21
    Article Snippet: BD Phosflow Lyse/Fix Buffer 5X (BD Biosciences) was used for red blood cell lysis and white blood cell fixation. .. Following fixation, white blood cells were washed with PBS (Rockland), resuspended in Cell Staining Buffer (Fluidigm), and stored at −80 °C. .. Prior to staining, samples were thawed at room temperature, washed with Cell Staining Buffer, and barcoded using the Cell-ID 20-Plex Pd Barcoding Kit (Fluidigm).

    Article Title: ProMPt: A modular preclinical platform for functional modelling of prostate cancer heterogeneity and therapeutic vulnerabilities
    Article Snippet: .. Cells were filtered (40 μm), counted then stained with a panel of metal-conjugated extracellular antibodies (Table S5) in Cell Staining Buffer (CSB: Standard BioTools, 201068) supplemented with TruStain FcX (BioLegend, 101320) at room temperature for 30 min on a rocker. .. Cells were then washed in CSB and fixed with 4% PFA for 20 min at room temperature, then permeabilised with FoxP3 Fix/Perm Buffer (Fisher Scientific, 11500597) for 30 min at 4°C, followed by 50% methanol for 10 min on ice.

    Article Title: Signalome-wide mapping of the NFκB pathway in T-cells reveals novel targets for immunotherapy
    Article Snippet: .. Cells were then washed in cell staining buffer (CSB; Standard BioTools, Cat# 201068) and stained with extracellular rare-earth metal-conjugated antibodies for 30 minutes at room temperature. ..

    Article Title: Phenoscaping Reveals Multimodal γδ T-cell Cytotoxicity as a Strategy to Overcome Cancer Cell–Mediated Immunomodulation
    Article Snippet: PDO-γδ T cells were dissociated into single cells using 0.875 mg mL −1 dispase II (Thermo Fisher Scientific, 17105041), 0.2 mg mL −1 collagenase IV (Thermo Fisher Scientific, 17104019), and 0.2 mg mL −1 DNase I (Sigma, DN25) in C-Tubes (Miltenyi, 130-096-334) via gentleMACS Octo Dissociator with Heaters (Miltenyi, 130-096-427, SCR_020271). .. Single PDO and γδ T cells were washed in cell staining buffer (Standard BioTools, 201068) and stained with extracellular rare-earth metal-conjugated antibodies for 30 minutes at room temperature. .. PDO–γδ T cells were then permeabilized in 0.1% (v/v) Triton X-100/PBS (Sigma, T8787) and 50% methanol/PBS (Thermo Fisher Scientific, 10675112) and stained with intracellular rare-earth metal-conjugated antibodies for 30 minutes at room temperature.

    Article Title: SPP1 + Tumor-Associated Macrophages Drive Immunotherapy Resistance via CD8 + T-cell Dysfunction in Clear-Cell Renal Cell Carcinoma
    Article Snippet: Cells were incubated with eBioscience Cell Stimulation Cocktail (1X, 2 μL/mL) for 5 hours. .. After centrifugation, cells were resuspended in cell staining buffer (201068, Fluidigm) for further staining. ..

    Article Title: Reversing the Warburg Effect: YW3-56 Induces Leukemia Differentiation via AKT-Mediated Glucose Metabolic Reprogramming
    Article Snippet: After incubation with different concentrations of YW3-56 for 24 h, the NB4 cells were collected and cultured with Cell-IDTM cisplatin (Fluidigm, South San Francisco, CA, USA) for 2 min to distinguish living cells. .. FIX I solution (Fluidigm) was then used to fix the cells for 15 min. After three washes with Cell Staining Buffer (CSB, Fluidigm), the cells were stained with a surface marker antibody cocktail for 30 min at room temperature. .. Following another three washes with CSB, the cells were permeabilized with Perm-S (Fluidigm), washed three times with CSB, and stained with an intracellular marker antibody cocktail.

    Article Title: Pre-treatment naïve T cells are associated with severe irAE following PD-1/CTLA4 checkpoint blockade for melanoma
    Article Snippet: Cells were stained for viability using 103Rh, followed by FC block (Invitrogen). .. Surface staining was done in cell staining buffer (Fluidigm) for 30 minutes followed by fixing and permeabilization with FoxP3/Transcription Factor Staining Buffer Set (Invitrogen) and barcoding (Fluidigm). ..

    Article Title: A randomized phase II study: CRS207/GVAX plus anti-PD1 and anti-CTLA4 recruits mesothelin- and mKRAS-specific T cells into PDAC
    Article Snippet: Cells were first stained for viability using platinum at a 1:1,000 dilution (Standard BioTools cat# 201064). .. After quenching in media and washing in cell staining buffer (CSB; Standard BioTools cat# 201068), cells were barcoded using a 5-choose-3 schema based on CD45 as previously described ( 37 ) for 25 minutes at RT. .. All wells were washed three times with CSB and multiplexed into flow tubes for Fc block (Human TruStain FcX TM , BioLegend cat# 422302) for 10 minutes, followed by a cocktail of surface antibodies for 30 minutes at RT ( Supplementary Table S2 ).

    Centrifugation:

    Article Title: SPP1 + Tumor-Associated Macrophages Drive Immunotherapy Resistance via CD8 + T-cell Dysfunction in Clear-Cell Renal Cell Carcinoma
    Article Snippet: Cells were incubated with eBioscience Cell Stimulation Cocktail (1X, 2 μL/mL) for 5 hours. .. After centrifugation, cells were resuspended in cell staining buffer (201068, Fluidigm) for further staining. ..

    Marker:

    Article Title: Reversing the Warburg Effect: YW3-56 Induces Leukemia Differentiation via AKT-Mediated Glucose Metabolic Reprogramming
    Article Snippet: After incubation with different concentrations of YW3-56 for 24 h, the NB4 cells were collected and cultured with Cell-IDTM cisplatin (Fluidigm, South San Francisco, CA, USA) for 2 min to distinguish living cells. .. FIX I solution (Fluidigm) was then used to fix the cells for 15 min. After three washes with Cell Staining Buffer (CSB, Fluidigm), the cells were stained with a surface marker antibody cocktail for 30 min at room temperature. .. Following another three washes with CSB, the cells were permeabilized with Perm-S (Fluidigm), washed three times with CSB, and stained with an intracellular marker antibody cocktail.



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